Minimum and maximum extracellular Ca2+ requirements during mouse sperm capacitation and fertilization in vitro

Sperm Capacitation and in Vitro Fertilization in Cattle

The slaughtered cows or heifers have a large number of immature oocytes within primordial follicles or developing follicles. Therefore, it is useful to aspirate immature oocytes, which would be wasted if left unprocessed, from ovaries; subsequently they are matured in vitro (IVM), fertilized in vitro (IVF), and cultured in vitro (IVC). Also most recently it has been shown that immature oocytes ...

Human sperm capacitation and in-vitro fertilization in a chemically defined and protein-free medium SMART1.

Media for sperm capacitation and in-vitro fertilization (IVF) are supplemented by proteins (albumin, globulins) extracted from human or animal sera, which raises the problem of potential contamination by pathogens. The present study aimed to evaluate the efficiency of a protein-free medium (SMART1, Bio-Media, Boussens, France) and to compare it with a human serum albumin (HSA) containing medium...

Changes in sperm plasma membrane lipid diffusibility after hyperactivation during in vitro capacitation in the mouse

We have used the technique of fluorescence recovery after photobleaching to measure the diffusibility of the fluorescent lipid analogue, 1,1'-dihexadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate on the morphologically distinct regions of the plasma membranes of mouse spermatozoa, and the changes in lipid diffusibility that result from in vitro hyperactivation and capacitation with bovi...

Sperm surface galactosyltransferase activities during in vitro capacitation

Studies using genetic and biochemical probes have suggested that mouse sperm surface galactosyltransferases may participate during fertilization by binding N- acetylglucosamine (GlcNAc) residues in the egg zona pellucida. In light of these results, we examined sperm surface galactosyltransferase activity during in vitro capacitation to determine whether changes in enzymatic activity correlated ...

Ca2+ Signaling During Mammalian Fertilization: Requirements, Players, and Adaptions